@article{oai:nitech.repo.nii.ac.jp:00005390,
 author = {Sakai, Tomoharu and Nakagawa, Hiroyuki and Kitagawa, Shinya and Ohtani, Hajime},
 issue = {6},
 journal = {Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
 month = {Jun},
 note = {Dual-gradient capillary electrochromatography (DG-CEC) was developed to provide superior performance with regard to the separation of ionized analytes; in this method, both the eluent composition and the applied voltage are varied during the separation procedure. As for the gradient in the eluent composition, a shift in the pH is employed to control not only the electrophoretic mobility, but also the retention factor of the analytes. The dual-gradient method was shown to be effective in increasing the resolution and reducing the chromatographic period of ionized analytes. Fourteen kinds of o-phthalaldehyde labeled amino acids were separated within 8 min using DG-CEC with multistage enlargement in the applied voltage. The separation efficiency increased particularly for highly retained amino acids in the dual-gradient, as compared to those in the ordinary single-gradient for the eluent., application/pdf},
 pages = {735--738},
 title = {Dual-Gradient Capillary Electrochromatography by Varying the Mobile Phase Composition and Applied Voltage},
 volume = {24},
 year = {2008}
}