{"created":"2023-05-15T12:36:13.538099+00:00","id":5450,"links":{},"metadata":{"_buckets":{"deposit":"113f7006-6cc1-4a8b-97fc-6a3a0f134e39"},"_deposit":{"created_by":91,"id":"5450","owners":[91],"pid":{"revision_id":0,"type":"depid","value":"5450"},"status":"published"},"_oai":{"id":"oai:nitech.repo.nii.ac.jp:00005450","sets":["31"]},"author_link":["8677","8633","19084","8677","19076","19077","19078","19079","19080","19081","8633","19083","19084"],"item_10001_biblio_info_28":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-06-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageEnd":"3111","bibliographicPageStart":"3102","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Organic & biomolecular chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Design methodology of variant proteins, in which original functions can be manipulated by additive ligand binding, is an attractive target of protein engineering. Especially for multi-protein complexes, techniques for constructing variants which allow the switching on or off of original functions by ligands have been limited until now. We examined a method of utilizing a de novo designed protein module, IZ-DS, which has a tertiary structure that can be significantly changed from a random coil to a folded coiled-coil structure following binding with peptide ligand, IZ-3K. By introducing a metamorphosis IZ-DS sequence to one of the components in a target multi-protein complex, the IZ-3K binding and the subsequent structural transition of the IZ-DS moiety would affect the tertiary structure of the introduced protein unit, and the function of the total multi-protein complex may also be altered. In this research, we used the T7 RNA polymerase (T7 RNAP)/T7 lysozyme complex as the target multi-protein complex, in which allosteric binding of the T7 lysozyme to T7 RNAP halts the RNA synthesis of T7 RNAP. The IZ-DS sequence was introduced to the T7 lysozyme. By optimizing the introduction site of the IZ-DS sequence in the T7 lysozyme, we succeeded in constructing the T7 lysozyme variant, DS-Lys23. In the absence of IZ-3K, the mixture of T7 RNAP and DS-Lys23 exhibited RNA synthesis due to the weakening of the interaction between T7 RNAP and DS-Lys23. Whereas, after the addition of IZ-3K, RNA synthesis was significantly suppressed by the binding of DS-Lys23/IZ-3K complex. The present methodology using a designed ligand-dependent metamorphosis protein sequence constitutes another possible method for the de novo manipulation of various functions of natural protein complexes.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_full_name_27":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"8677","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090345950","nameIdentifierScheme":"NRID","nameIdentifierURI":"http://rns.nii.ac.jp/nr/1000090345950"}],"names":[{"name":"水野, 稔久"}]},{"nameIdentifiers":[{"nameIdentifier":"8633","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070202033","nameIdentifierScheme":"NRID","nameIdentifierURI":"http://rns.nii.ac.jp/nr/1000070202033"}],"names":[{"name":"神取, 秀樹"}]},{"nameIdentifiers":[{"nameIdentifier":"19084","nameIdentifierScheme":"WEKO"}],"names":[{"name":"田中, 俊樹"}]}]},"item_10001_publisher_29":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Royal Society of Chemistry","subitem_publisher_language":"en"}]},"item_10001_relation_34":{"attribute_name":"item_10001_relation_34","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1039/b901118h"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1039/b901118h","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_30":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1477-0520","subitem_source_identifier_type":"ISSN"}]},"item_10001_source_id_32":{"attribute_name":"item_10001_source_id_32","attribute_value_mlt":[{"subitem_source_identifier":"AA1168650X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_33":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mizuno, Toshihisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8677","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090345950","nameIdentifierScheme":"NRID","nameIdentifierURI":"http://rns.nii.ac.jp/nr/1000090345950"}]},{"creatorNames":[{"creatorName":"Suzuki, Kumiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19076","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19077","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitade, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furutani, Yuji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kudo, Motonori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kandori, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8633","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070202033","nameIdentifierScheme":"NRID","nameIdentifierURI":"http://rns.nii.ac.jp/nr/1000070202033"}]},{"creatorNames":[{"creatorName":"Tsumoto, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19083","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Toshiki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19084","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-25"}],"displaytype":"detail","filename":"OBC 7_3102.pdf","filesize":[{"value":"437.0 kB"}],"format":"application/pdf","license_note":"Org. Biomol. Chem., 2009, 7, 3102-3111-Reproduced by permission of The Royal Society of Chemistry (RSC)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文_fulltext","url":"https://nitech.repo.nii.ac.jp/record/5450/files/OBC 7_3102.pdf"},"version_id":"43d127a7-69e9-45c5-a2ef-b98c9b9c1397"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Manipulation  of protein-complex function by using an engineered heterotrimeric coiled-coil switch","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Manipulation  of protein-complex function by using an engineered heterotrimeric coiled-coil switch","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"91","path":["31"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-11-06"},"publish_date":"2012-11-06","publish_status":"0","recid":"5450","relation_version_is_last":true,"title":["Manipulation  of protein-complex function by using an engineered heterotrimeric coiled-coil switch"],"weko_creator_id":"91","weko_shared_id":-1},"updated":"2025-03-21T03:05:19.438807+00:00"}